FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11442209
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC345420.3018822676635255TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG342900.2996798957264284TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41900.040.6679421
TATGCCG46750.039.32377245
ATCGGAA88750.022.9028631
TCGGAAG89000.022.5531222
CGCGTAT602.4683643E-422.50137141
CGCCAAT85650.022.30268132
ACGCCAA85400.022.18383631
GTCACGC85200.022.0508628
TCTCGTA83400.021.82668140
CTCGTAT83250.021.81214141
TATCTCG85100.021.78622238
CGTATGC84650.021.77085343
CGTCTGA87150.021.71170615
TCGTATG84650.021.69082342
CGGAAGA90150.021.6886583
ACACGTC86950.021.5022312
AGTCACG86500.021.4337127
ATCTCGT86700.021.35822139
ACGTCTG89850.021.00918614
GCACACG93050.020.52775810
Produced by FastQC (version 0.11.5)