FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683256
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGC688970.469221540508454TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82450.040.9874245
CGTCCCG89400.037.90209634
GTCCCGA90700.037.21000335
CCCGATC90550.036.8491137
ATCGGAA94100.036.837011
ACACGTC93300.036.77570312
TCCCGAT91350.036.772736
CTCGTAT91450.036.6834143
TCGGAAG94150.036.6675032
CCGATCT90800.036.6487838
TCTCGTA92000.036.39073642
ACCCGTC93450.036.37910531
CACCCGT94150.036.18044330
CGTCTGA94600.036.1739615
ATCTCGT92550.036.12585441
TCGTATG93800.035.90828744
TCACCCG94600.035.86563529
CGGAAGA96900.035.602333
CCGTCCC95500.035.5741533
CCCGTCC96350.035.35420232
Produced by FastQC (version 0.11.5)