FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960074
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC351180.27097067501312105TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC42350.040.6922845
ATCGGAA46500.037.887211
CTCGTAT45950.037.11251443
GTCACGA46750.037.00550528
TCGGAAG47700.036.8962822
TCTCGTA44450.036.18881242
TCGTATG47400.036.11960244
TATCTCG44900.035.9761640
ACACGTC48600.035.96454212
ACGAGTG48150.035.92870731
AGTCACG48300.035.81795527
CGTCTGA48750.035.76159315
CACGAGT48650.035.42072730
ATCTCGT45800.035.02319341
CGGAAGA50500.034.939683
ACGTCTG50750.034.44091814
CACGTCT52450.033.41040413
GCACACG55050.032.07860610
CGAGTGG55100.031.3972332
CACACGT56250.031.07396111
Produced by FastQC (version 0.11.5)