FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394110
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG143950.19468198336243306TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC91790.124139348751912TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11650.037.6825831
TATGCCG18100.037.54217545
ATCGGAA27850.025.2209451
CGTATGC28000.024.3485343
CTCGTAT28600.023.83836641
ACACGTC29100.023.81264712
TCTCGTA28750.023.63460740
TCGGAAG30000.023.4047362
CGTCTGA29750.023.36815515
TCGTATG29300.023.19142742
CGGAAGA30300.023.1730043
ATCTCGT30300.022.57424439
ACGTCTG31100.022.20894414
GCACACG32750.021.50220510
CACGTCT32250.021.48676113
GTATGCC33250.020.84251644
CACACGT34250.020.56050111
GATCTCG37300.018.39811338
GTCACAC41000.017.0113828
AGCACAC45400.015.758739
Produced by FastQC (version 0.11.5)