FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6643327
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG91570.13783756241413375TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12600.038.93383445
CGTATGC14650.033.48071343
ATCGGAA14900.033.402391
CTCGTAT14900.032.76721241
TCTCGTA14400.031.71683140
TCGTATG15750.030.99928942
TCGGAAG16200.030.9873732
ACACGTC16300.030.0897512
ATCTCGT15950.028.91674439
CGTCTGA17350.028.39842815
CGGAAGA17750.028.1554583
ACGTCTG17950.027.57451614
GATCGGA3900.027.1396851
CACGTCT18750.026.15802213
GCACACG19300.025.64553510
AATCTCG18450.025.24237438
GTATGCC20200.024.61966344
CACACGT20350.024.54341311
AGAGCAC28200.018.1905237
AGTCACC28400.017.9829327
Produced by FastQC (version 0.11.5)