FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458196
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1302.775596E-819.0397345
GATCGGA1102.2327004E-618.4316141
TCTCGTA2251.039527E-612.00160741
CTCGTAT2158.207453E-611.51316941
ATGCCCG3358.076313E-1011.41815934
CGTATGC2452.8991217E-611.02126544
ATCGGAA2301.7372815E-510.760622
ACGTCTG3205.7783836E-810.54532914
CGTTGGA8550.010.2757141
TCGTATG2709.2471455E-610.00133942
TATCTCG2504.3471526E-59.90143738
AACGGGA8700.09.3092948
CCGGCTA6350.09.21393638
AAACGGG10600.09.12632757
CACGTCT4051.8500396E-78.88758713
ATCTCGT3351.2344088E-58.73251240
ACCGCGC3351.2356328E-58.73182731
ACGCCCG2350.00270766448.617208534
ACACGTC3401.4932313E-58.6016812
GAAACGG10500.08.5705576
Produced by FastQC (version 0.11.5)