FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8166363
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG132580.16234889387111495TruSeq Adapter, Index 22 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC106900.13090282663163516TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15600.039.6705671
CGTATGC14500.036.46401245
CGTACGT26500.023.26304634
CCGTACG26500.023.17814433
ACCGTAC27500.022.33530232
CTCGTAT27400.022.25273144
ATCGGAA28300.022.023542
TCTCGTA26100.021.98467643
GTACGTA28000.021.85610435
TCGGAAG28700.021.7160593
CGTAATC26400.021.56424938
ACACGTC28850.021.36826713
TCGTATG28650.021.28184345
CGTCTGA28950.021.2167416
CACCGTA29250.020.99900231
ACGTAAT27600.020.7897337
TACGTAA29700.020.52932736
GTCACCG29950.020.50971629
ATCTCGT28050.020.4563342
ACGTCTG30100.020.33138515
Produced by FastQC (version 0.11.5)