FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16131116
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7550.026.22961445
CGCCAAT10250.019.75320232
GTCACGC11800.018.49356728
CTCGTAT11750.017.23707441
ACGCCAA12250.017.0791331
CGTATGC12400.016.6963843
ACACGTC12900.016.3925912
TCTCGTA10600.016.34721440
TATCTCG11050.015.67653138
TCGTATG13150.015.57298342
GATCGGA6600.015.40162851
AGTCACG15150.014.84972227
ATCGGAA14250.014.7423321
CGTCTGA15200.014.3562715
TCGGAAG15450.013.6883752
CGTTGGA36900.013.5289481
ATCTCGT13900.012.62784639
ACGGGAT24850.012.5839039
AACGGGA32450.012.5484318
ACGTCTG17600.012.39859614
Produced by FastQC (version 0.11.5)