FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117999085
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1431610.1213238221296377TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC1407510.11928143341111501TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA190600.036.0180471
CGTATGC206050.034.85870745
ACGTCCG337650.021.25186331
CGTCCGC342150.021.07776632
GTCCGCA349100.020.46526733
CTCGTAT351400.020.28735443
TCCGCAC354750.020.13958234
TCTCGTA354600.020.0663642
ATCGGAA365700.019.701711
TCGTATG362900.019.6374944
TCGGAAG374650.019.2649942
CGCACAT369950.019.22147836
ACACGTC378550.019.12494712
ATCTCGT373350.019.06463641
CCGCACA376550.018.92032435
GTCACGT381150.018.8376728
AGTCACG378100.018.59085727
CGTCTGA390850.018.54035615
CATCTCG387350.018.37558240
CGGAAGA396500.018.2600653
Produced by FastQC (version 0.11.5)