FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5497265
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG14500.012.1015577
CGTTGGA14050.011.8618451
CGGCCTA2106.312752E-611.78794739
AACGGGA13200.011.7595268
ACGGGAT9700.011.3642039
GAAACGG15050.011.0613946
GTTGCGT1450.006833421510.8613630
GGAAACG15650.010.6373155
CGGGATT9800.010.1003710
GATTGCG3053.9211132E-58.8510766
ATTGCGC2801.5039176E-48.8379057
GCCACCG14050.08.80781628
CCCTCGT2050.0085646048.7888681
ACGCCCG4152.6760426E-78.67571134
CGCACCC3401.48868585E-58.60386733
CGCCCGG8250.08.18277335
TCGGTTT2750.00122319738.181213
CGTTAAT2500.0046948038.1077321
CGGGAAT6155.14774E-108.04744910
ACCGCGC5352.3477696E-87.990350231
Produced by FastQC (version 0.11.5)