FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12628202
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG10923198.649837878741566TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC7911026.264565612745187TruSeq Adapter, Index 9 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGCCG269580.21347457064750786TruSeq Adapter, Index 9 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGCCG221820.1756544597560286TruSeq Adapter, Index 9 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGCCG156170.12366764484761965TruSeq Adapter, Index 9 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA869250.044.8108631
TATGCCG1241200.044.22690645
ACTCGTA44500.029.57986840
GCTCGTA39850.028.85313440
AGCTCGT41100.027.86500739
AACTCGT48650.026.82429939
GAGCTCG43650.026.5464338
ATGCCGT23300.026.3599945
ATCGGAA2098700.026.3591731
TCGGAAG2100800.026.3292522
CGGAAGA2102150.026.3049163
ACACGTC2096900.026.24121912
CGTCTGA2095350.026.2179615
ACGTCTG2097750.026.21020914
CACACGT2105950.026.20643411
CACGTCT2100700.026.20124613
GCACACG2104400.026.18821710
CGTATGC2098900.026.16248343
CTCGTAT2095450.026.15616841
AGTCACG2105950.026.13451827
Produced by FastQC (version 0.11.5)