FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7931076
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG130720.1648200067683124TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16650.039.1876345
ATCGGAA18250.036.6242181
CGTATGC17800.036.28236843
CTCGTAT17850.036.0544441
TCGGAAG18900.035.1216352
TCTCGTA16000.034.73824740
CGTCTGA19050.034.36849215
ACACGTC19300.034.2723812
TCGTATG19200.033.63677642
ATCTCGT16850.032.8523339
CGGAAGA20400.032.758533
ACGTCTG21000.031.28427314
CACGTCT22100.029.93074813
GCACACG22450.029.56433710
GTATGCC24150.027.11072244
CACACGT24500.027.0905911
GATCTCG25300.021.96885538
GTCACAC32650.020.39773828
AGAGCAC36200.019.019787
AGCACAC37500.018.4191519
Produced by FastQC (version 0.11.5)