FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5530927
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6450.034.53678545
CGTTTCG6200.034.1107732
CTCGTAT6700.032.57347543
ATCGGAA7100.031.4025061
TCTCGTA6550.030.91521542
TCGTATG7150.030.52615744
GTTTCGG7200.029.99785433
TTCGGAA7500.029.99785435
ACACGTC7700.029.21816312
GTCACGT7500.028.49821928
CGTCTGA8100.028.33130615
ATCTCGT7700.027.4665641
AGTCACG7950.027.45111327
TTTCGGA8100.026.66475934
TCGGAAT7600.026.64307236
ACGTCTG8500.026.46821614
TCGGAAG8450.026.3731272
CGGAATC7900.025.91610337
TCACGTT8300.025.75140429
CACGTCT9150.024.83384113
Produced by FastQC (version 0.11.5)