FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11719843
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8650.026.26792545
CTCGTAT8650.024.44748143
TCTCGTA8350.023.70939342
ATCGGAA9950.022.280841
ACACGTC10500.021.2100812
TCGTATG10200.021.17353844
CGTCTGA11050.019.95113615
AGTCACG11050.019.74797427
TCGGAAG11150.019.5803852
AACGATC10600.018.6764237
GTCACGT11800.018.30215828
ATCTCGT11000.017.79268641
CGGAAGA13350.017.0280323
ACGTCTG13200.016.70151914
CACGTCT14850.015.60296613
TCACGTG14550.015.15202229
CGTGAAA14450.014.94564332
AAACGAT13450.014.88622336
GCACACG15650.014.80562310
ACGTGAA14850.014.54300631
Produced by FastQC (version 0.11.5)