FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811091
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG8600.037.3580831
CGTCCGC8700.036.1542632
CGTATGC9200.035.4110145
TCTCGTA8050.034.88889742
GTCCGCA9000.034.69978333
CTCGTAT9200.033.94602243
ATCGGAA9800.033.9427571
ACACGTC9750.033.1824612
TCCGCAC9450.033.1116634
CGTCTGA9950.032.28967315
TCGTATG9700.032.1959544
TCGGAAG10300.032.071592
ATCTCGT9100.030.61555941
CGCACAT9000.030.48511736
GTCACGT10850.030.44060128
AGTCACG10750.030.30576127
CACGTCC10950.030.16182730
CGGAAGA11300.029.2328933
CCGCACA10900.028.706935
TCACGTC11350.028.70369329
Produced by FastQC (version 0.11.5)