FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459546
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG137720.1846224957926394TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19200.039.6128645
CTCGTAT19350.039.1868741
CGTATGC20300.037.57719843
ATCGGAA20850.037.4898031
TCTCGTA17850.037.05960540
TCGTATG20900.036.6060942
ACACGTC21700.035.66608412
ATCTCGT18550.035.4178239
TCGGAAG22100.035.2243842
CGTCTGA21800.035.193115
CGGAAGA23050.033.9676133
ACGTCTG22600.033.84777514
CACGTCT23900.032.4771513
GCACACG24950.031.29072610
GTATGCC25100.030.6600744
GCGAACG300.005146834530.0026442
CACACGT26750.029.18518311
GTCACAC31600.024.42199928
GATCTCG27850.023.59068538
AGAGCAC34250.023.1227137
Produced by FastQC (version 0.11.5)