FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23664995
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9900.012.27163945
CTCGTAT9800.011.93766643
CGTTGGA55750.011.7266351
ACGGGAT37700.010.6823259
ATCGGAA11200.010.4665991
AAACGGG62950.010.3647137
AACGGGA50200.010.0840028
CGGGATT36650.010.00618710
GGAAACG67350.09.7210295
GAAACGG66600.09.6277896
TCTCGTA9700.09.277801542
TCGGAAG13350.09.2711922
TCGTATG13200.09.20372944
AACGGGT16700.08.7569128
ACACGTC14650.08.44620712
CGTCTGA15450.08.15446115
CGGAAGA17700.08.1369343
GTCACGT15350.07.914490728
ACGGGTT16800.07.6334299
ACCGCGC18600.07.49927231
Produced by FastQC (version 0.11.5)