FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9082943
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG899390.9901966796444721TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC139050.15308914742721605TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA98500.043.69631
CTCGTAT114300.036.7507644
GTCACGA114850.036.67493429
TCTCGTA114750.036.56883243
TCGTATG115450.036.54099345
CGTCTGA116650.036.3762616
ATCGGAA118500.036.30682
TATCTCG115750.036.2336641
ACGAGTG116350.036.16463532
CGTATGC21450.036.08312245
ATCTCGT116400.036.06979442
CGGAAGA119000.036.0589144
TCGGAAG119350.036.0474363
CACGAGT116650.036.0330531
ACACGTC117500.035.9786813
AGTCACG116900.035.85915828
ACGTCTG118850.035.7403715
CACGTCT121400.034.97111514
CACACGT121450.034.82723612
GCACACG122450.034.6723911
Produced by FastQC (version 0.11.5)