FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25750550
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16750.021.20441645
CTCGTAT17000.020.6263643
ATCGGAA18050.019.5735571
TCTCGTA15350.019.32913842
TCGTATG19000.018.57346744
ACACGTC21450.016.76606812
TCGGAAG21350.016.7583542
AGTCACG21200.016.53994227
GTCACGT21450.016.13758928
CGTCTGA21450.016.13733915
AACGATC19050.015.78736337
CGGAAGA25500.014.4689223
ATCTCGT21500.013.69577241
ACGTCTG26500.013.57102414
CGTTGGA60300.012.3899081
GAAACGA30100.012.32106135
CGTGAAA29100.012.05018532
CACGTGA30550.011.91971630
CACGTCT30950.011.76502513
AAACGAT27200.011.63908536
Produced by FastQC (version 0.11.5)