FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16438865
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG20000.035.88180532
CGTACGT20300.035.24146333
CGTATGC21950.033.8329345
ACCGTAC22500.032.39485531
GTACGTA23850.030.08993134
CTCGTAT25350.028.67376943
TCTCGTA21650.028.27295742
TCGTATG26350.028.01260444
CACCGTA26050.027.98037330
ATCGGAA26900.027.9671481
GTCACCG27000.027.82984728
TCGGAAG27400.027.5886252
ACACGTC28200.026.88370312
CGTAATC22350.026.77460137
CGTCTGA29700.025.67758815
ATCTCGT24300.025.18969241
CGGAAGA31000.024.6739523
TACGTAA29650.024.65911735
ACGTCTG31750.023.87795414
ACGTAAT25800.023.5430636
Produced by FastQC (version 0.11.5)