FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005310911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263102391
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4601805317.490549145180516TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA208976177.942769702917675TruSeq Adapter, Index 2 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9944360.3779653982696037TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9813670.3729981306023175TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9636650.3662699515338118TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9180570.3489352554002445TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9179710.34890256850611445TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9091700.34555748298007677TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9064230.3445134027687342TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8724130.33158687638076234TruSeq Adapter, Index 2 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7813960.2969931200663243TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7619940.28961880471850215TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6883230.26161791893407765TruSeq Adapter, Index 2 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC5598560.21279016046646268TruSeq Adapter, Index 2 (100% over 62bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4915940.18684512829075733TruSeq Adapter, Index 2 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4548480.17287870257325028TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4437830.16867311555522885TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4414280.16777802676829343TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4296720.16330980435673806TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4177340.1587724073552794TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4157140.15800464542338577TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4055730.154150252477181TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4003760.15217497586329423TruSeq Adapter, Index 2 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3960650.15053645027498058TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3496050.1328779258414265TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3265830.12412772029882464TruSeq Adapter, Index 2 (98% over 62bp)
ACCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3224490.12255646889959278TruSeq Adapter, Index 2 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)