FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005310913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252299961
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6486899225.711059067504177TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2990290711.852125098029642TruSeq Adapter, Index 2 (100% over 62bp)
ACCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15833640.6275720351776035TruSeq Adapter, Index 2 (98% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA13942990.5526354401616416TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA13714960.5435973888240118TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA13469010.5338490718197139TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12870510.5101273083431035TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12794660.5071209662216317TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12694560.5031534665992279TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12561120.4978645240456458TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12183110.482881961285757TruSeq Adapter, Index 2 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10706440.42435361295993224TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10590350.4197523439173263TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9406340.3728236803017183TruSeq Adapter, Index 2 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6458390.25598061824512136TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6311070.25014153688275836TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6275950.24874954300924368TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6105930.24201073895528663TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5897600.23375350422666138TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5891600.23351569206148232TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC5769460.2286746290856541TruSeq Adapter, Index 2 (100% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5693050.22564609116209894TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5687920.22544276176087083TruSeq Adapter, Index 2 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5497510.21789579269891365TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4914960.194806213228071TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4581750.18159931463485243TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA2774840.10998178473757274TruSeq Adapter, Index 2 (100% over 62bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2548400.10100675362371538TruSeq Adapter, Index 2 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)