FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005310915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369324299
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10348935028.021267563551238TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7479502220.2518551317957TruSeq Adapter, Index 2 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC27080250.7332377012106642TruSeq Adapter, Index 2 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA22072600.5976481931940254TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA22056180.5972035974811395TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21619110.5853692827289438TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20602540.5578441509476743TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20573310.5570527055951983TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20550180.5564264267377652TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20220570.547501749945784TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA19573440.529979750939702TruSeq Adapter, Index 2 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17642080.4776853309616652TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17069750.46218865225545314TruSeq Adapter, Index 2 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16373110.44332609699206393TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15778380.42722290525487466TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15657520.42395044253505776TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15381330.4164721910160587TruSeq Adapter, Index 2 (98% over 62bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15287100.41392077481476514TruSeq Adapter, Index 2 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14915860.4038689043853029TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14886780.40308152050401647TruSeq Adapter, Index 2 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14561840.3942832908484042TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA14389790.38962478339395695TruSeq Adapter, Index 2 (100% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14324120.3878466713071592TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14298910.387164073382564TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA12560300.3400886438831364TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA11639700.3151620413689596TruSeq Adapter, Index 2 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC9036390.24467358428533834TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA9019690.2442214071595652TruSeq Adapter, Index 2 (100% over 62bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5584180.15119990791615906TruSeq Adapter, Index 2 (98% over 63bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3735950.1011563552713871TruSeq Adapter, Index 2 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)