FastQCFastQC Report
Fri 27 May 2022
EGAF00005310923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382659263
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA233139986.092626065607616TruSeq Adapter, Index 5 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA48556641.268926292789102TruSeq Adapter, Index 5 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC16845650.4402258517912841TruSeq Adapter, Index 5 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA6536980.17083030863413334TruSeq Adapter, Index 5 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5222040.13646710023585656TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5000810.13068571660318073TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4823690.12605705562130873TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4710110.12308887972744566TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4647350.12144877830907232TruSeq Adapter, Index 5 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4488630.1173009628673225TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4404000.11508933471185827TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4339920.1134147378525631TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4233170.11062504973256063TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4132700.10799947628603467TruSeq Adapter, Index 5 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3990120.10427344600828337TruSeq Adapter, Index 5 (98% over 62bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)