FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005310927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397758849
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12591715631.656657373322194TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA182226494.581330885739766TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA27935780.7023295665258725TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA27347670.6875439746659162TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA26479490.6657171818193792TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA26206520.6588544809470726TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25526530.6417589467632435TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25287070.6357387161485878TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA24357060.6123574638561969TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA24074800.6052612043836642TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23949580.6021130657485385TruSeq Adapter, Index 15 (97% over 39bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA22311910.5609406316438733TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA21851340.5493615052169462TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC8794550.22110256056176392TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8706810.21889670140311573TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8036890.20205433569122178TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7095050.1783756670112448TruSeq Adapter, Index 15 (97% over 39bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5006970.12587953762909246TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4896370.12309895838420429TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4037870.1015155290737479TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4024810.10118718942692838TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)