FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005310929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382900670
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6883890817.978267836407806TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA160094234.181090359544161TruSeq Adapter, Index 16 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15732910.4108875024950988TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15650820.40874360444446334TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15339390.40061016346615425TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15303790.39968041842287716TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14695230.3837870014695979TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14498430.3786472872977736TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14492070.3784811867788061TruSeq Adapter, Index 16 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14297360.3733960559536237TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14125220.36890037303930545TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14066630.36737021118296814TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13925560.36368596586681345TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13202200.34479438231330334TruSeq Adapter, Index 16 (97% over 39bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12448870.32512008923880964TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12438630.32485265695669846TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11949710.3120838101432416TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11368940.29691616888526207TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC10825390.28272058129331556TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7923340.20692938458425786TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4837760.1263450387799008TruSeq Adapter, Index 16 (97% over 39bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4043850.10561094082180635TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)