FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39282483
Sequences flagged as poor quality0
Sequence length75
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG685390.1744772600041601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACT150.00235724468.991776
GGATCTC309.135938E-657.495412
ACCTCTC309.138626E-657.4925585
TCCAGCG200.0073575351.7558969
TGAGCCC306.316508E-445.996332
ATCCCCT405.0087416E-543.119644
AAACCCC1100.040.7686963
TGAATCC350.001348892339.4254262
AATCCGC350.00134901939.4246753
AACCCCC702.1947926E-839.423674
ACGCCTC350.001349221639.423475
ACCCTCC350.001349221639.423475
AGGCCCC350.001349221639.423475
AAAGACC801.6443664E-938.8134081
ATCCCTC557.4069685E-637.6316874
AATCCAC400.002598638434.496593
AGCCCCT501.8639574E-434.4955375
ATTCCCC851.2112105E-732.4673773
CCTCTCT652.325366E-531.8423566
AAATCCC1052.3370376E-829.569072
Produced by FastQC (version 0.11.5)