FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35641458
Sequences flagged as poor quality0
Sequence length75
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG624240.1751443501553724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCAG150.002357160268.9923810
TCGCACA200.007358985551.7532968
GATTCCC200.00736282451.7464642
GAACCCT200.007363395851.7454453
ACCCTTC200.007363722751.744875
AGACCCG352.275454E-549.2813763
ATGCGCC306.3167565E-445.9959533
ACTGAAC400.002596102234.50345265
AATCCCG400.002598496934.4969643
TAACGCC400.002598496934.4969643
ATGCCCG501.8635005E-434.4969643
TACCACC400.002598639834.496584
AACGCCC501.8636235E-434.496584
TAATCCC751.6719878E-632.19782
ATCCCCT450.004626778430.6636224
ATCCCCC450.004626778430.6636224
ATATCCC802.7666138E-630.1848433
ACGCCCC802.7668557E-630.1845075
CCCTCCA605.422874E-428.7523169
AAACGCC605.4282194E-428.7474693
Produced by FastQC (version 0.11.5)