FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31091451
Sequences flagged as poor quality0
Sequence length75
%GC20

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA252.5676016E-455.20272469
TGTGCAC200.007356485451.7577218
TCCGCAG306.312817E-446.0017666
TGGACGT350.00134803239.4305269
GTAGCTC501.8616035E-434.5029367
GTTGGAG450.004622606630.66926867
TCTACGG450.00462366230.66783766
GTTGATC802.762874E-630.1900667
GTTAGTC500.00773476527.60234367
AGCGTGG500.00773610127.60136269
CTGCCGA500.00773610127.60136269
GAAGGCC500.00773634427.60118765
AGTACTG500.007736404427.60114164
TCGCGGC500.007736404427.60114164
GTCGAAG658.6596946E-426.53977469
CACGCAG750.001993890423.00058263
ACAGTAG953.0669486E-421.79069168
TCGTCGG1154.605605E-521.00117368
GACTAAG1004.3382242E-420.70005862
TCTCGAG1056.0277176E-419.7143462
Produced by FastQC (version 0.11.5)