FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34515031
Sequences flagged as poor quality0
Sequence length75
%GC20

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC200.007360291751.75096566
GCAGTGC200.007364088751.7442138
GTCGGCT350.001348189839.42959269
GAGACGT400.002597045634.50089369
GAATGGT450.00462394730.6674669
CACGTTG450.00462411130.66723865
TACTGCG500.0077356927.60167367
TGGTAGC500.0077356927.60167367
ACTGCGT500.00773667527.60095268
CTCGTGA700.00133415924.64435267
GTCGCCG851.4438912E-424.35441867
CTTGGAG851.4441468E-424.35378368
TTTCGAG750.00199229323.00376153
AGGTTAG902.1277205E-423.00059369
GGTCGCC750.001993972823.00042766
GGAGATC1253.5070625E-622.08076368
AGCGTAC1004.3365444E-420.70125467
ACAGTCG1004.337308E-420.70071468
ATGGTAG900.005735710319.1678367
TAAGGTA900.00574037119.16513459
Produced by FastQC (version 0.11.5)