FastQCFastQC Report
Fri 27 May 2022
EGAF00005310941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400410985
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA181567654.53453218822156TruSeq Adapter, Index 4 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA91026402.2733242445883444TruSeq Adapter, Index 4 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC15657650.3910394716069041TruSeq Adapter, Index 4 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA5003660.12496310509563069TruSeq Adapter, Index 4 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)