FastQCFastQC Report
Fri 27 May 2022
EGAF00005310943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389542124
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA151142033.879991936379132TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA58528051.502483207695402TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC11710540.30062320038076296TruSeq Adapter, Index 6 (100% over 62bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4281530.10991186154748184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)