FastQCFastQC Report
Tue 31 May 2022
EGAF00005310957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394269449
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA34355070.8713601849480354TruSeq Adapter, Index 14 (97% over 43bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860320.14863743601904086No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4743290.12030579625255215TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)