FastQCFastQC Report
Fri 27 May 2022
EGAF00005310961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394204499
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA52449401.3305124658153635TruSeq Adapter, Index 14 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6801230.17253050173838833TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6313830.16016636075987556TruSeq Adapter, Index 14 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5711250.14488038605566497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)