FastQCFastQC Report
Fri 27 May 2022
EGAF00005310965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393166530
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA46874011.1922177098849183TruSeq Adapter, Index 14 (97% over 43bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5898140.15001633023034794No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5590910.14220208419063546TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4870930.12388974208969415TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)