FastQCFastQC Report
Sat 25 May 2024
EGAF00005310968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211340770
Total Bases15.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5759567527.252514978534432Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG102630494.856161449586844Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCCGT11298680.5346190420333947Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT11016250.5212553167095966Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCCGT10821330.5120322974123734Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT10722660.5073635342579664Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCCGT10569380.5001107926312561Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT10000730.4732040107547635Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT9685890.4583067431806935Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT6107450.2889858875786248Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCATGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3512830.1662163907134435Illumina Single End PCR Primer 1 (98% over 50bp)
NTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2527060.11957276393002637Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCCG2117360.10018701076938445Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)