FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005310977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218868263
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA191028088.727993605907129TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA33589941.5347104024853526TruSeq Adapter, Index 14 (97% over 44bp)
ACCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23401791.069218062008378TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4807730.21966318616052616TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3998120.1826724416412991TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3789140.1731242322693446TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3737480.1707639083332973TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3725070.17019690058946554TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3623590.1655603215528786TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3593400.16418095299636934TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3555490.1624488608474039TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3528330.16120793173197523TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3358130.15343156444751427TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3278320.14978507870736835TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3154830.14414287191560524TruSeq Adapter, Index 14 (97% over 40bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2761990.12619417553471424TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)