FastQCFastQC Report
Mon 30 May 2022
EGAF00005310982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414107847
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGGGG29394910.7098370681200833Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGGGGG4690290.11326252409797972Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGG4208030.10161676554755071Illumina Single End PCR Primer 1 (100% over 56bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)