FastQCFastQC Report
Fri 27 May 2022
EGAF00005310983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410510525
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA369801559.008332977577128TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA61363191.494801868965479TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13560990.33034451430934686TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9012320.21953931631838183TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8688720.21165644900334774TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8025120.1954912118270293TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7761010.18905751563860634TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7673850.18693430576475475TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7526770.18335145000240857TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7500850.18272004110004245TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7185750.17504423303154043TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7147650.17411612040884944TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6671560.1625186102110293TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6670330.16248864752006054TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6652220.16204748952539036TruSeq Adapter, Index 13 (97% over 39bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5845610.1423985414259476TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)