FastQCFastQC Report
Fri 27 May 2022
EGAF00005310985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409912664
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4755566811.601414685739009TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA154416703.7670634152449605TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10606620.2587531669916887TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10500370.2561611514398101TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10423590.2542880695191208TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10042700.24499609019154384TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9924960.24212377102845495TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9912640.24182321920163952TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9743480.2376964864886438TruSeq Adapter, Index 14 (97% over 40bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9422270.2298604270494068TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9310580.22713570030127198TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9141470.2230101873603007TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9137080.22290309137655723TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8869110.21636584518891563TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8476710.20679307434131872TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8191570.19983695844049357TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC6228310.15194236594749364TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)