FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44727968
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT22242834.972913144634695TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTA12377152.7672059683104764TruSeq Adapter, Index 23 (97% over 37bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2441360.5458240356458849Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1231490.2753288501726705Illumina Single End PCR Primer 1 (100% over 33bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT837410.18722290268138272TruSeq Adapter, Index 7 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC777630.17385766328575444Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA639320.14293517648733786Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA600860.1343365296630511Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAAACTCGTAT505970.11312161554041533TruSeq Adapter, Index 23 (97% over 37bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTA467250.10446483953842928TruSeq Adapter, Index 23 (97% over 37bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACG459900.10282157239962254Illumina Single End PCR Primer 1 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)