FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46138959
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT9655292.0926544961710123TruSeq Adapter, Index 5 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTA7215801.5639277860603662TruSeq Adapter, Index 5 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC565070.12247133707546372Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)