FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48426458
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTAT492579310.171697876396411TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTA26157175.401421264384028TruSeq Adapter, Index 2 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC4788240.9887652737270193Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC2101410.43393840614979523Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA1014230.2094371634613459Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC911430.18820909842301495Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACG674440.13927097455692505Illumina Single End PCR Primer 1 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAACCTCGTAT653330.13491178727133007TruSeq Adapter, Index 2 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA612510.12648251086214068Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAAGCTCGTAT596680.12321363664466231TruSeq Adapter, Index 2 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC525150.10844278555330229Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG521420.10767254545025778Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)