FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49524763
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTAT8459921.708220188756885TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTA5929581.197295987060049TruSeq Adapter, Index 10 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC640280.12928481858661292Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)