FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35924573
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTAT15120584.208979742083504TruSeq Adapter, Index 10 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTA7249412.0179530039229694TruSeq Adapter, Index 10 (97% over 39bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1520610.42327851746491185Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTAT652800.1817140596215298TruSeq Adapter, Index 4 (97% over 35bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA608720.16944390682110544Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC558830.1555564766211696Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA502540.13988753603278736Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC477500.13291737663799094Illumina Single End PCR Primer 1 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)