FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52698089
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTAT26727395.071794918407763TruSeq Adapter, Index 2 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTA19714833.741090118087584TruSeq Adapter, Index 2 (97% over 40bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2299110.4362795774245248Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1537980.29184739507347224Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA941830.17872185080563358Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC559880.10624294175069612Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA538830.10224848950405013Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)