FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41130603
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTAT10918822.654670538139205TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTA6802351.653841544700913TruSeq Adapter, Index 1 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC942340.22910921096877668Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC551320.13404131225598612Illumina Single End PCR Primer 1 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)