FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46299379
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT25175075.437453059575594TruSeq Adapter, Index 16 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTA22976944.9626885924323085TruSeq Adapter, Index 16 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2109440.4556087026566814Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1496460.3232138383540738Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA839140.1812421717362559Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACACCTCGTAT560460.12105129962974234TruSeq Adapter, Index 16 (97% over 38bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC503570.10876387780492693Illumina Single End PCR Primer 1 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACACCTCGTA494890.10688912263812436TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACAACTCGTAT494500.10680488824698924TruSeq Adapter, Index 16 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)