FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36714712
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT15626774.256269257947604TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTA7977052.1727121269533587TruSeq Adapter, Index 7 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1444800.3935207227010251Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA564210.15367409119265324Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA422180.11498932634961156Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC400110.10897811209849609Illumina Single End PCR Primer 1 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)