FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57268392
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA3953920.6904192455761636TruSeq Adapter, Index 5 (97% over 36bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG3254690.5683222256353907No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT2551500.4455337247813768TruSeq Adapter, Index 5 (97% over 36bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1798820.31410345867577355No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1565650.2733881544989075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)